Abstract
Filamentous actinobacteria of the genus Streptomyces produce a great variety of
secondary metabolites, and are the primary sources of antibacterial compounds in clinical use.
Although Streptomyces have been screened since 1950, their diverse secondary metabolism still
represents a potential source of new natural products. Also, unexplored habitats and the
development of new isolation and cultivation methods promise the discovery of new Streptomyces
species. This study aimed for the isolation and determination of antimicrobial and anticancer
properties, as well as mass spectrometric characterization of secondary metabolites produced
by newly isolated Streptomyces strains. Furthermore, the influence of stimulants (e.g. rare earth
elements and N-acetylglucosamine) on the activation, enhance or inhibition of antibiotic
production was also investigated. Fifty-nine Streptomyces isolates were recovered from four soil
and compost samples in Austria and identified by multilocus sequence analysis. The antimicrobial
activity of Streptomyces isolates was tested against multiple indicator strains, including Grampositive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli, Klebsiella
pneumoniae) and yeast (Candida albicans). Ten percent of the isolates inhibited the growth of at
least one indicator strain. The total crude extract from the culture supernatant of the four most
promising bioactive isolates were tested for anticancer activity against two cancer cell lines,
human hepatoma HepG2 cells and human breast cancer cell line MCF-7. One isolate exhibited
strong cytotoxicity activity against both cancer cell lines. According to the multilocus sequence
analysis of four genes (16s rRNA, rpoB, atpD and recA), this isolate may represent a new
Streptomyces species. Relevant secondary metabolites in the bioactive crude extracts were
analyzed by untargeted LC-UV-MS (high performance liquid chromatography coupled with UV
detection and high-resolution mass spectrometry). Here, we show first results derived from two
different cultivation approaches (not induced (NI) and with N-Acetylglucosamine (GlcNAc)
induced) and suggest relevant m/z values of putative metabolites, which are necessary for the
further identification steps. Our results indicate that the bioactive metabolites of four
Streptomyces isolates can potentially be used therapeutically, and they will be investigated
further.
secondary metabolites, and are the primary sources of antibacterial compounds in clinical use.
Although Streptomyces have been screened since 1950, their diverse secondary metabolism still
represents a potential source of new natural products. Also, unexplored habitats and the
development of new isolation and cultivation methods promise the discovery of new Streptomyces
species. This study aimed for the isolation and determination of antimicrobial and anticancer
properties, as well as mass spectrometric characterization of secondary metabolites produced
by newly isolated Streptomyces strains. Furthermore, the influence of stimulants (e.g. rare earth
elements and N-acetylglucosamine) on the activation, enhance or inhibition of antibiotic
production was also investigated. Fifty-nine Streptomyces isolates were recovered from four soil
and compost samples in Austria and identified by multilocus sequence analysis. The antimicrobial
activity of Streptomyces isolates was tested against multiple indicator strains, including Grampositive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli, Klebsiella
pneumoniae) and yeast (Candida albicans). Ten percent of the isolates inhibited the growth of at
least one indicator strain. The total crude extract from the culture supernatant of the four most
promising bioactive isolates were tested for anticancer activity against two cancer cell lines,
human hepatoma HepG2 cells and human breast cancer cell line MCF-7. One isolate exhibited
strong cytotoxicity activity against both cancer cell lines. According to the multilocus sequence
analysis of four genes (16s rRNA, rpoB, atpD and recA), this isolate may represent a new
Streptomyces species. Relevant secondary metabolites in the bioactive crude extracts were
analyzed by untargeted LC-UV-MS (high performance liquid chromatography coupled with UV
detection and high-resolution mass spectrometry). Here, we show first results derived from two
different cultivation approaches (not induced (NI) and with N-Acetylglucosamine (GlcNAc)
induced) and suggest relevant m/z values of putative metabolites, which are necessary for the
further identification steps. Our results indicate that the bioactive metabolites of four
Streptomyces isolates can potentially be used therapeutically, and they will be investigated
further.
| Original language | English |
|---|---|
| Number of pages | 1 |
| Publication status | Published - Sept 2023 |
| Event | 15th ÖGMBT ANNUAL MEETING - NAWI-University of Salzburg Duration: 19 Sept 2023 → 21 Sept 2023 https://vwgoe.at/events/15th-oegmbt-annual-meeting-september-19-21-2023-at-nawi-university-of-salzburg/ |
Conference
| Conference | 15th ÖGMBT ANNUAL MEETING |
|---|---|
| Period | 19/09/23 → 21/09/23 |
| Internet address |