Generation of CDMLe012-A-1 cells: A pluripotent human embryonic stem cell model of Turner's syndrome

Aleksey Y Domozhirov; John L Mazzilli; Rick A Wetsel; Eva M Zsigmond

doi:10.1016/j.scr.2019.101508

Generation of CDMLe012-A-1 cells: A pluripotent human embryonic stem cell model of Turner's syndrome

Aleksey Y Domozhirov
, John L Mazzilli
, Rick A Wetsel
, Eva M Zsigmond

The University of Texas Health Science Center at Houston
The Brown Foundation Institute of Molecular Medicine
McGovern Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

Monosomy of chromosome X is associated with high prenatal mortality of female embryos and severe developmental abnormalities of patients born with Turner's syndrome (45,XO). The CDMLe012-A-1 human embryonic stem cell (hESC) line, derived from a day six blastocyst with a normal 46,XX female karyotype spontaneously lost an X-chromosome during cell culture. This 45,XO karyotype was stably maintained for more than 55 passages. Since the CDMLe012-A-1 cells express pluripotent stem cell markers and differentiate into cells derived from the three germ layers, the cell line represents a stable, pluripotent stem cell model of Turner's syndrome.

Original language	English
Article number	101508
Pages (from-to)	101508
Journal	Stem Cell Research
Volume	39
DOIs	https://doi.org/10.1016/j.scr.2019.101508
Publication status	Published - Aug 2019
Externally published	Yes

Keywords

Blastocyst/cytology
Cell Differentiation/genetics
Female
Human Embryonic Stem Cells/cytology
Humans
Karyotype
Karyotyping
Pregnancy
Turner Syndrome/genetics

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10.1016/j.scr.2019.101508

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title = "Generation of CDMLe012-A-1 cells: A pluripotent human embryonic stem cell model of Turner's syndrome",

abstract = "Monosomy of chromosome X is associated with high prenatal mortality of female embryos and severe developmental abnormalities of patients born with Turner's syndrome (45,XO). The CDMLe012-A-1 human embryonic stem cell (hESC) line, derived from a day six blastocyst with a normal 46,XX female karyotype spontaneously lost an X-chromosome during cell culture. This 45,XO karyotype was stably maintained for more than 55 passages. Since the CDMLe012-A-1 cells express pluripotent stem cell markers and differentiate into cells derived from the three germ layers, the cell line represents a stable, pluripotent stem cell model of Turner's syndrome.",

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year = "2019",

month = aug,

doi = "10.1016/j.scr.2019.101508",

language = "English",

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journal = "Stem Cell Research",

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T1 - Generation of CDMLe012-A-1 cells

T2 - A pluripotent human embryonic stem cell model of Turner's syndrome

AU - Domozhirov, Aleksey Y

AU - Mazzilli, John L

AU - Wetsel, Rick A

AU - Zsigmond, Eva M

PY - 2019/8

Y1 - 2019/8

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AB - Monosomy of chromosome X is associated with high prenatal mortality of female embryos and severe developmental abnormalities of patients born with Turner's syndrome (45,XO). The CDMLe012-A-1 human embryonic stem cell (hESC) line, derived from a day six blastocyst with a normal 46,XX female karyotype spontaneously lost an X-chromosome during cell culture. This 45,XO karyotype was stably maintained for more than 55 passages. Since the CDMLe012-A-1 cells express pluripotent stem cell markers and differentiate into cells derived from the three germ layers, the cell line represents a stable, pluripotent stem cell model of Turner's syndrome.

KW - Blastocyst/cytology

KW - Cell Differentiation/genetics

KW - Female

KW - Human Embryonic Stem Cells/cytology

KW - Humans

KW - Karyotype

KW - Karyotyping

KW - Pregnancy

KW - Turner Syndrome/genetics

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DO - 10.1016/j.scr.2019.101508

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SN - 1873-5061

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JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101508

ER -

Generation of CDMLe012-A-1 cells: A pluripotent human embryonic stem cell model of Turner's syndrome

Abstract

Keywords

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