Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells

John L Mazzilli; Aleksey Y Domozhirov; Stacey L Mueller-Ortiz; Charles A Garcia; Rick A Wetsel; Eva M Zsigmond

doi:10.1016/j.scr.2016.12.005

Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells

John L Mazzilli
, Aleksey Y Domozhirov
, Stacey L Mueller-Ortiz
, Charles A Garcia
, Rick A Wetsel
, Eva M Zsigmond

The Brown Foundation Institute of Molecular Medicine
McGovern Medical School
The University of Texas Health Science Center at Houston

Research output: Contribution to journal › Article › peer-review

Abstract

The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.

Original language	English
Pages (from-to)	37-40
Number of pages	4
Journal	Stem Cell Research
Volume	18
DOIs	https://doi.org/10.1016/j.scr.2016.12.005
Publication status	Published - Jan 2017
Externally published	Yes

Keywords

Animals
Blastocyst/cytology
Cell Differentiation
Cell Line
Female
Human Embryonic Stem Cells/cytology
Humans
Karyotype
Mice
Mice, Inbred NOD
Mice, SCID
Microscopy, Fluorescence
Phagocytosis
Retinal Pigment Epithelium/cytology
Teratoma/metabolism
Transcription Factors/genetics

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10.1016/j.scr.2016.12.005

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title = "Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells",

abstract = "The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.",

keywords = "Animals, Blastocyst/cytology, Cell Differentiation, Cell Line, Female, Human Embryonic Stem Cells/cytology, Humans, Karyotype, Mice, Mice, Inbred NOD, Mice, SCID, Microscopy, Fluorescence, Phagocytosis, Retinal Pigment Epithelium/cytology, Teratoma/metabolism, Transcription Factors/genetics",

author = "Mazzilli, \{John L\} and Domozhirov, \{Aleksey Y\} and Mueller-Ortiz, \{Stacey L\} and Garcia, \{Charles A\} and Wetsel, \{Rick A\} and Zsigmond, \{Eva M\}",

year = "2017",

month = jan,

doi = "10.1016/j.scr.2016.12.005",

language = "English",

volume = "18",

pages = "37--40",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

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T1 - Derivation and characterization of the human embryonic stem cell line CR-4

T2 - Differentiation to human retinal pigment epithelial cells

AU - Mazzilli, John L

AU - Domozhirov, Aleksey Y

AU - Mueller-Ortiz, Stacey L

AU - Garcia, Charles A

AU - Wetsel, Rick A

AU - Zsigmond, Eva M

PY - 2017/1

Y1 - 2017/1

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AB - The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.

KW - Animals

KW - Blastocyst/cytology

KW - Cell Differentiation

KW - Cell Line

KW - Female

KW - Human Embryonic Stem Cells/cytology

KW - Humans

KW - Karyotype

KW - Mice

KW - Mice, Inbred NOD

KW - Mice, SCID

KW - Microscopy, Fluorescence

KW - Phagocytosis

KW - Retinal Pigment Epithelium/cytology

KW - Teratoma/metabolism

KW - Transcription Factors/genetics

UR - https://www.scopus.com/pages/publications/85028040144

U2 - 10.1016/j.scr.2016.12.005

DO - 10.1016/j.scr.2016.12.005

M3 - Article

C2 - 28395800

SN - 1873-5061

VL - 18

SP - 37

EP - 40

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells

Abstract

Keywords

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