TY - CONF
T1 - Click Chemistry Modification of Metal Nanoparticles for targeted SERS
AU - Seidl, Stefan
AU - Zimmermann, Daniel
AU - Lilek, David
AU - Rudolf-Scholik, Judith
AU - Herbinger, Birgit
AU - Prohaska, Katerina
PY - 2023
Y1 - 2023
N2 - Raman spectroscopy is a promising method for the analysis of biological materials, but it is held back by the inherent weak intensity of the Raman signals. By bringing metal nanoparticles into close contact with the biological material, the signal intensity can be increased by several orders of magnitude [1]. Usually, the contact between biological material and nanoparticle happens at random points, with nanoparticle solution and cells being mixed and measured. In contrast, probes made from antibodies and nanoparticles can be manufactured to allow for targeted measurements, with the antibody binding to antigens on the surface or in the cytosol of cells. Three approaches for the modification of the nanoparticles are used. In the first approach, nanoparticles are coated in streptavidin using unspecific and non-covalent interactions. The streptavidin on the surface can then bind biotinylated antibodies, creating a probe [2]. In the second approach, the nanoparticle and the antibody are modified with reactive groups. These groups are then joined together using copper catalyzed azide alkyne click chemistry [3] . In the third approach, a bivalent polyethylene glycol linker binds to the antibody and the nanoparticle. Different protocols for the synthesis of gold nanoparticles have been evaluated. The necessary amounts of streptavidin and BSA for the conjugation have been determined by titration and a first conjugate of nanoparticle, streptavidin and BSA has been successfully produced. As part of the RaDiaM project, these conjugates will be used to enhance Raman signals in melanoma cells, to distinguish them from healthy precursor cells and cells treated with cytostatic [4].
AB - Raman spectroscopy is a promising method for the analysis of biological materials, but it is held back by the inherent weak intensity of the Raman signals. By bringing metal nanoparticles into close contact with the biological material, the signal intensity can be increased by several orders of magnitude [1]. Usually, the contact between biological material and nanoparticle happens at random points, with nanoparticle solution and cells being mixed and measured. In contrast, probes made from antibodies and nanoparticles can be manufactured to allow for targeted measurements, with the antibody binding to antigens on the surface or in the cytosol of cells. Three approaches for the modification of the nanoparticles are used. In the first approach, nanoparticles are coated in streptavidin using unspecific and non-covalent interactions. The streptavidin on the surface can then bind biotinylated antibodies, creating a probe [2]. In the second approach, the nanoparticle and the antibody are modified with reactive groups. These groups are then joined together using copper catalyzed azide alkyne click chemistry [3] . In the third approach, a bivalent polyethylene glycol linker binds to the antibody and the nanoparticle. Different protocols for the synthesis of gold nanoparticles have been evaluated. The necessary amounts of streptavidin and BSA for the conjugation have been determined by titration and a first conjugate of nanoparticle, streptavidin and BSA has been successfully produced. As part of the RaDiaM project, these conjugates will be used to enhance Raman signals in melanoma cells, to distinguish them from healthy precursor cells and cells treated with cytostatic [4].
M3 - Poster
ER -