@article{fe05a110141b4919a46723a900bd685d,
title = "Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells",
abstract = "The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.",
keywords = "Animals, Blastocyst/cytology, Cell Differentiation, Cell Line, Female, Human Embryonic Stem Cells/cytology, Humans, Karyotype, Mice, Mice, Inbred NOD, Mice, SCID, Microscopy, Fluorescence, Phagocytosis, Retinal Pigment Epithelium/cytology, Teratoma/metabolism, Transcription Factors/genetics",
author = "Mazzilli, \{John L\} and Domozhirov, \{Aleksey Y\} and Mueller-Ortiz, \{Stacey L\} and Garcia, \{Charles A\} and Wetsel, \{Rick A\} and Zsigmond, \{Eva M\}",
note = "Copyright {\textcopyright} 2016 The Authors. Published by Elsevier B.V. All rights reserved.",
year = "2017",
month = jan,
doi = "10.1016/j.scr.2016.12.005",
language = "English",
volume = "18",
pages = "37--40",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier B.V.",
}