Calcium transport from mineral waters across Caco-2 cells

In the present study the absorption of calcium from 13 different mineral waters has been examined. For this purpose the mineral waters were first digested in vitro by simulating gastric and intestinal digestion. Afterward, the absolute and fractional transport rates of calcium from these digested solutions across human colon adenocarcinoma (Caco-2) cell monolayers grown on bicameral filters were measured. Results showed that the fractional transport rates for calcium lie between 1.65 and 6.72% after 90 min of incubation time. The absolute transport values varied between 90.4 and 624.7 pmol/(min·cm2). The transport values [pmol/(min·cm2)] for calcium from the mineral waters were in general not concentration dependent, showing neither signs of saturation kinetics nor unsaturable uptake mechanisms. In addition, the fractional transport rates from mineral waters with similar calcium concentrations were greatly different in some cases. On the basis of these results, it can be concluded that calcium bioavailability from mineral waters could vary dependent on probably several factors.